عنوان

معرفی مواد گزینشگر مناسب برای انتقال ژن به گیاه دارویی نعناع فلفلی (.‮‭Mentha piperita L‬)

Number

‭۲۴۱پ‬

.Language of Text, Soundtrack etc

per

Title Proper

معرفی مواد گزینشگر مناسب برای انتقال ژن به گیاه دارویی نعناع فلفلی (.‮‭Mentha piperita L‬)

First Statement of Responsibility

/شیرین بهرام پور

Name of Publisher, Distributor, etc.

: کشاورزی

Specific Material Designation and Extent of Item

‮‭۱۱۱‬ص‬

Text of Note

جدول، نمودار، عکس

Text of Note

چاپی

Text of Note

واژه نامه بصورت زیرنویس

Text of Note

کتابنامه ص.: ‮‭۱۰۳-۱۱۱‬

Dissertation or thesis details and type of degree

کارشناسی ارشد

Discipline of degree

مهندسی کشاورزی- بیوتکنولوژی

Date of degree

‮‭۱۳۸۵/۰۶/۲۵‬

Body granting the degree

تبریز

Text of Note

.Efficiency of transformation methods is due to precise, simple and quick identification of transformed and nontransformed tissues. To determine a suitable selective system has an important role in success of plant transformation. In this research, the effect of streptomycin and kanamycin antibiotics, glyphosate herbicide and a toxic compound called phenyl mercuric acetate (PMA) as selective agents were studied. For different concentrations and plant samples including leaf, leaf disk, shoot and callus, separate experiments were done for each selective agent. Plant samples were provided from field, greenhouse and in vitro cultures. The experiments were analyzed in as a split plot in time based on completely randomized design. Moreover, for quantification of the variation due to the effect of the antibiotics on plant samples, chlorophyll a, b and carotenoid were measured. Analysis of variance (ANOVA) showed a difference significant at 0.01 level for different PMA concentrations during studied days on shoot, leaf disk and leaf explants of peppermint. Mean comparison results showed that 2 to 4 and 1.5 ppm concentrations would cause plant death, in shoot and leaf disk explants, respectively. This result was obtained in the concentration of 1.5 ppm during 10 days. ANOVA of other selective agents containing kanamycin , streptomycin and glyphosate showed significant interactions, as well. Kanamycin concentration 100 ppm in shoot and bud samples caused whiteness of apexes and lateral buds and finally, leaf death. The sterptomysin concentration of 250 ppm was the optimal dose. Glyphosate concentration of 10000 and 2000 ppm caused necrosis and death of shoot and leaf samples during 2-3 and 6-9 days, respectively. Influence of this agent on leaf disks had different pattern in the field and greenhouse conditions. Studying the effect of the selective agents on callus, showed that using callus to produce transgenic plants seemed not to be feasible, so other methods such as morphogenesis, organogenesis and direct embryogenesis may be more effective. Analysis of variance of different streptomycin concentrations was significant at 0.01 level for photosynthetic characters including chlorophyll a, b, total chlorophyll, chlorophyll ratio a/b and carotenoid. Changes in mean values of these treatments for all the pigments showed decreasing trend while to increasing the streptomycin concentration. Results of assessing photosynthetic pigments for kanamycin were similar to those for streptomycin

بهرام پور، شیرین

باغبان کهنه روز، بهرام، استاد راهنما

مطلبی آذر، علیرضا، استاد مشاور

زهتاب سلماسی، سعید، استاد مشاور

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