عنوان

YAC protocols /

شابک

1280832452

شابک

1588296121

شابک

1597451584

شابک

9781280832451

شابک

9781588296122

شابک

9781597451581

شماره

b791730

عنوان اصلي

YAC protocols /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Alasdair MacKenzie.

وضعيت ويراست

2nd ed.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©2006.

نام خاص و کميت اثر

1 online resource (xv, 203 pages) :

ساير جزييات

illustrations

عنوان فروست

Methods in molecular biology,

مشخصه جلد

349

شاپا ي ISSN فروست

1064-3745 ;

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Markers, selection, and media in yeast artificial chromosomes cloning / David Markie -- Construction of yeast artificial chromosomes libraries from pathogens and nonmodel organisms / Cecilia P. Sanchez and Michael Lanzer -- Analysis and screening of yeast artificial chromosomes libraries by filter hybridization / Cecilia P. Sanchez and Michael Lanzer -- Analysis of yeast artificial chromosome DNA by restriction digestion, southern blotting nucleic acid hybridization and polymerase chain reaction / Sylvia Vasiliou and John Peter Quinn -- Postgenomic bioinformatic analysis of yeast artificial chromosomes sequence / Kerry Miller, Scott Davidson, and Alasdair MacKenzie -- Transformation of yeast using bio-active beads with surface-immobilized yeast artificial chromosomes / Shigeki Kawakami [and others].

متن يادداشت

Modification and amplification of yeast artificial chromosomes / Sanbing Shen, Anthony Harmar, and Nick Hastie -- Modifying yeast artificial chromosomes to generate Cre/LoxP and FLP/FRT site-specific deletions and inversions / Gabriela G. Loots -- Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes / Natalay Kouprina, Vladimir N. Noskov, and Vladimir Larionov -- A polymerase chain reaction-mediated yeast artificial chromosomes-splitting technology for generating targeted yeast artificial chromosomes subclones / Yeon-Hee Kim [and others] -- Genomic reconstruction by serial mitotic recombination of yeast artificial chromosomes / David Markie, Emma Jones, and Jiannis Ragoussis -- Targeting vector construction by yeast artificial chromosome modification / Peter J. Murray.

متن يادداشت

Production of yeast artificial chromosomes transgenic mice by pronuclear injection of one-cell embryos / Alasdair MacKenzie -- Generation of yeast artificial chromosomes transgenic mice by intracytoplasmic sperm injection / Pedro N. Moreira [and others] -- Establishment of cell lines that exhibit correct ontogenic stage-specific gene expression pprofiles from tissues of yeast artificial chromosome transgenic mice using chemically induced growth signals / C. Anthony Blau and Kenneth R. Peterson -- Application of yeast artificial chromosomes in fluorescence in situ hybridization / Thomas Liehr -- Contribution of yeast artificial chromosome-based physical maps to the final assembly of the trypanosoma cruzi genome / Márcia R.M. Santos [and others].

بدون عنوان

0

بدون عنوان

0

بدون عنوان

0

متن يادداشت

It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.

منبع سفارش / آدرس اشتراک

Springer

شماره انبار

978-1-58829-612-2

عنوان

YAC protocols.

شماره استاندارد بين المللي کتاب و موسيقي

1588296121

موضوع مستند نشده

Genetic vectors.

موضوع مستند نشده

Molecular cloning, Methods.

موضوع مستند نشده

Saccharomyces cerevisiae.

موضوع مستند نشده

Yeast fungi-- Genetic engineering.

موضوع مستند نشده

Analytical, Diagnostic and Therapeutic Techniques and Equipment

موضوع مستند نشده

Anatomy.

موضوع مستند نشده

Cells.

موضوع مستند نشده

Cellular Structures.

موضوع مستند نشده

Chromosomes, Artificial, Yeast.

موضوع مستند نشده

Chromosomes, Artificial.

موضوع مستند نشده

Chromosomes, Fungal.

موضوع مستند نشده

Chromosomes.

موضوع مستند نشده

Fungal Structures.

موضوع مستند نشده

Genetic Phenomena.

موضوع مستند نشده

Genetic Structures.

موضوع مستند نشده

Genetic Techniques.

موضوع مستند نشده

Genetic Vectors.

موضوع مستند نشده

Investigative Techniques

موضوع مستند نشده

Phenomena and Processes

موضوع مستند نشده

Genetic vectors.

موضوع مستند نشده

Molecular cloning.

موضوع مستند نشده

Saccharomyces cerevisiae.

موضوع مستند نشده

SCIENCE-- Biotechnology.

موضوع مستند نشده

Yeast fungi-- Genetic engineering.

موضوع مستند نشده

PSB

موضوع مستند نشده

SCI-- 010000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

نشانه اثر

مستند نام اشخاص تاييد نشده

MacKenzie, Alasdair.

تاريخ عمليات

20201207050116.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y