عنوان

SV40 protocols /

شابک

0896036537

شابک

1280820926

شابک

1592591175

شابک

6610820929

شابک

9780896036536

شابک

9781280820922

شابک

9781592591176

شابک

9786610820924

شماره

b796066

عنوان اصلي

SV40 protocols /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Leda Raptis.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

2001.

نام خاص و کميت اثر

1 online resource (xi, 310 pages) :

ساير جزييات

illustrations

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 165

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Propagation of wild-type and mutant SV40 -- Propagation and assay of the JC virus -- Propagation and assay of BK virus -- Molecular methods for identification and genotyping of BK virus -- Methods for studying interactions between simian virus 40 T-antigen and the viral origin of replication -- Cell-free transcription of SV40 -- Quantitation and structural analysis of SV40 RNAs -- Generation of recombinant SV40 vectors for gene transfer -- Production of SV40 proteins in insect cells and in vitro packaging of virions and pseduovirions -- Using retroviral vectors to express SV40 tumor antigens -- Neoplastic transformation assays -- Methods to assess inhibition of adipocyte dfferentiation by SV40 large T antigen -- SV40-mediated immortalization -- Biology of p53 and SV40 large T association -- Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins -- In situ electroporation for the measurement of c-Ras activation by SVLT -- Role of SV40 small t in cell lysis, transformation, and signaling -- Cytotoxic T lymphocytes in SV40 infections -- Detection of SV40 DNA sequences in human tissue -- Guide to techniques in creating transgenic mouse models using SV40 T antigen.

بدون عنوان

0

متن يادداشت

Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.

شماره انبار

springerp

عنوان

SV40 protocols

عنوان اصلي به زبان ديگر

Simian virus 40 protocols

موضوع مستند نشده

SV40 (Virus), Laboratory manuals.

موضوع مستند نشده

Genetic Techniques.

موضوع مستند نشده

Simian virus 40-- genetics.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Biology.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Microbiology.

موضوع مستند نشده

SV40 (Virus)

موضوع مستند نشده

PSG.

موضوع مستند نشده

SCI-- 008000

موضوع مستند نشده

SCI-- 045000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

نشانه اثر

مستند نام اشخاص تاييد نشده

Raptis, Leda.

تاريخ عمليات

20201207090000.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y