عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304257557
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Characterization of cell populations isolated from different skeletal sites of adult rats
نام عام مواد
[Thesis]
نام نخستين پديدآور
P. Denkovski
نام ساير پديدآوران
J. N. Heersche
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Toronto (Canada)
تاریخ نشرو بخش و غیره
1995
مشخصات ظاهری
نام خاص و کميت اثر
112
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
M.Sc.
کسي که مدرک را اعطا کرده
University of Toronto (Canada)
امتياز متن
1995
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Clinical observations in primary hyperparathyroidism, corticosteroid-induced osteoporosis, and in osteoporotic patients treated with fluoride, progesterone or estrogen, suggest that different skeletal sites respond differently in various disease states and to their treatments. We hypothesized that these different response patterns could be based, at least in part, on differences in the properties on the populations of cells in these different sites. Cell populations derived from vertebral, femoral cancellous, femoral cortical and calvarial bone tissue were characterized histochemically by staining for alkaline phosphatase, tartrate-resistant acid phosphatase, Sudan IV, Alcian blue and usd\alphausd-napthylbutyrate esterase activity. Analysis of the cells subcultured from explants cultured for 14-16 days showed that, when compared to calvarial populations, the absolute cyclic adenosine monophosphate response to parathyroid hormone was 1/3 lower in vertebral, 2/3 lower in femoral cancellous and 1/3 lower in femoral cortical populations. The parathyroid hormone response was found to be lower than the prostaglandin E2 response in calvarial and femoral cancellous populations, but slightly higher in vertebral and femoral cortical populations. In addition, we analyzed and characterized the osteoprogenitors in these populations using a colony assay for osteoprogenitors previously developed for fetal rat calvarial cell populations (Bellows et al., 1986). The results show that cells isolated for explants cultured for 14-16 days from vertebral, femoral cancellous, calvarial and to a much lesser extent femoral cortical populations contain osteoprogenitors that differentiate to form bone modules in the presence of dexamethasone. Progenitors forming bone nodules in response to progesterone were most numerous in vertebral cells and calvarial populations, were detected in lower numbers in femoral cancellous populations and were infrequent in femoral cortical populations. The parathyroid hormone induced cyclic adenosine monophosphate response in calvarial and femoral cancellous day 22-24 cultures increased relative to day 14-16 derived cultures. (Abstract shortened by UMI.)
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Cellular biology
موضوع مستند نشده
Cellular biology
موضوع مستند نشده
Health and environmental sciences
موضوع مستند نشده
Pharmacology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
J. N. Heersche
مستند نام اشخاص تاييد نشده
P. Denkovski
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
