Investigation of nanoscopic dynamics and potentials by interferometric scattering microscopy /
نام عام مواد
[Book]
نام نخستين پديدآور
Jaime Ortega Arroyo.
وضعیت نشر و پخش و غیره
محل نشرو پخش و غیره
Cham, Switzerland :
نام ناشر، پخش کننده و غيره
Springer,
تاریخ نشرو بخش و غیره
2018.
مشخصات ظاهری
نام خاص و کميت اثر
1 online resource (xxxvi, 142 pages) :
ساير جزييات
illustrations (some color)
فروست
عنوان فروست
Springer theses
يادداشت کلی
متن يادداشت
"Doctoral thesis accepted by the University of Oxford, Oxford, UK."
یادداشتهای مربوط به کتابنامه ، واژه نامه و نمایه های داخل اثر
متن يادداشت
Includes bibliographical references.
یادداشتهای مربوط به مندرجات
متن يادداشت
Intro; Supervisor's Foreword; Abstract; Parts of this thesis have been published in the following journal articles:; Acknowledgements; Contents; List of Figures; 1 Introduction; References; 2 Non-fluorescent Single-Molecule Approaches to Optical Microscopy; 2.1 Introduction; 2.2 Single-Particle Tracking; 2.3 Scattering Detection: An Alternative to Fluorescence; 2.4 Interferometric Scattering; 2.4.1 Confocal Detection; 2.4.2 Non-scanned Wide-Field Detection; 2.4.3 Confocal Beam Scanning Wide-Field Detection; 2.5 Applications; 2.5.1 Lateral Single-Particle Tracking
متن يادداشت
2.5.2 Axial Localisation via Interferometry2.5.3 Label-Free Imaging; 2.6 Conclusion and Outlook; References; 3 Experimental Methods; 3.1 Experimental Optics and Hardware; 3.1.1 Interferometric Scattering Channel; 3.1.2 Focus Control Feedback Channel; 3.1.3 Single-Molecule Fluorescence Channel; 3.1.4 Sample Stage Stabilisation; 3.1.5 Camera Characterisation; 3.1.6 Operation and Synchronisation of the Acousto-Optic Beam Deflector; 3.1.7 Data Acquisition; 3.2 Experimental iSCAT Microscopy; 3.2.1 Image Processing; 3.2.2 Spot Detection; 3.2.3 Localisation; 3.2.4 Trajectory Linking
4.3.4 Recovery of Brownian Motion upon Tuning Substrate Interactions and Interleaflet Coupling4.4 Discussion; 4.4.1 Importance of Simultaneous Localisation Precision and Time Resolution; 4.4.2 Thermal and Optical Force Considerations; 4.4.3 Membrane Defects, Labelling Artefacts, and CTxB Induced Aggregation Do Not Cause Transient Binding; 4.4.4 Transient Binding Requires Substrate Interaction and Interleaflet Coupling; 4.4.5 Multiple CTxB-GM1 Interactions Result in Ring-Like Structures; 4.4.6 A Model of Transient Binding: Molecular Pinning; 4.5 Conclusion and Outlook; References
متن يادداشت
5 Structural Dynamics of Myosin 5a5.1 Introduction; 5.2 Experimental Methods; 5.2.1 Sample Preparation; 5.2.2 Experimental Setup; 5.3 Experimental Results; 5.3.1 N-Terminus Labelling Does Not Perturb the Kinetics of Myosin 5a; 5.3.2 During Myosin Movement the Motor Domain Undergoes a Transition Between Two Distinct States; 5.3.3 The Labelled Motor Domain Moves in Three Dimensions; 5.3.4 A Conformational Change in the Motor Domain Accompanies the Power Stroke; 5.3.5 Myosin Steps via a Single, Spatially-Constrained Transient State
بدون عنوان
0
بدون عنوان
8
بدون عنوان
8
بدون عنوان
8
بدون عنوان
8
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physics to molecular biology and synthetic organic chemistry. Especially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field.
یادداشتهای مربوط به سفارشات
منبع سفارش / آدرس اشتراک
Springer Nature
شماره انبار
com.springer.onix.9783319770956
ویراست دیگر از اثر در قالب دیگر رسانه
عنوان
Investigation of nanoscopic dynamics and potentials by interferometric scattering microscopy.
شماره استاندارد بين المللي کتاب و موسيقي
9783319770949
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Interferometry.
موضوع مستند نشده
Microscopy.
موضوع مستند نشده
Atomic & molecular physics.
موضوع مستند نشده
Cellular biology (cytology)
موضوع مستند نشده
Interferometry.
موضوع مستند نشده
Microscopy.
موضوع مستند نشده
NATURE-- Reference.
موضوع مستند نشده
Organic chemistry.
موضوع مستند نشده
Physical chemistry.
موضوع مستند نشده
SCIENCE-- Life Sciences-- Biology.
موضوع مستند نشده
SCIENCE-- Life Sciences-- General.
مقوله موضوعی
موضوع مستند نشده
NAT-- 027000
موضوع مستند نشده
SCI-- 008000
موضوع مستند نشده
SCI-- 086000
رده بندی ديویی
شماره
570
.
28/2
ويراست
23
رده بندی کنگره
شماره رده
QH205
.
2
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )