عنوان
پدید آورنده
موضوع
رده
QH205.2
QH205
.
2
کتابخانه
محل استقرار
INTERNATIONAL STANDARD BOOK NUMBER
(Number (ISBN
3319770950
(Number (ISBN
9783319770956
Erroneous ISBN
3319770942
Erroneous ISBN
9783319770949
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
Investigation of nanoscopic dynamics and potentials by interferometric scattering microscopy /
General Material Designation
[Book]
First Statement of Responsibility
Jaime Ortega Arroyo.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Cham, Switzerland :
Name of Publisher, Distributor, etc.
Springer,
Date of Publication, Distribution, etc.
2018.
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
1 online resource (xxxvi, 142 pages) :
Other Physical Details
illustrations (some color)
SERIES
Series Title
Springer theses
GENERAL NOTES
Text of Note
"Doctoral thesis accepted by the University of Oxford, Oxford, UK."
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references.
CONTENTS NOTE
Text of Note
Intro; Supervisor's Foreword; Abstract; Parts of this thesis have been published in the following journal articles:; Acknowledgements; Contents; List of Figures; 1 Introduction; References; 2 Non-fluorescent Single-Molecule Approaches to Optical Microscopy; 2.1 Introduction; 2.2 Single-Particle Tracking; 2.3 Scattering Detection: An Alternative to Fluorescence; 2.4 Interferometric Scattering; 2.4.1 Confocal Detection; 2.4.2 Non-scanned Wide-Field Detection; 2.4.3 Confocal Beam Scanning Wide-Field Detection; 2.5 Applications; 2.5.1 Lateral Single-Particle Tracking
Text of Note
2.5.2 Axial Localisation via Interferometry2.5.3 Label-Free Imaging; 2.6 Conclusion and Outlook; References; 3 Experimental Methods; 3.1 Experimental Optics and Hardware; 3.1.1 Interferometric Scattering Channel; 3.1.2 Focus Control Feedback Channel; 3.1.3 Single-Molecule Fluorescence Channel; 3.1.4 Sample Stage Stabilisation; 3.1.5 Camera Characterisation; 3.1.6 Operation and Synchronisation of the Acousto-Optic Beam Deflector; 3.1.7 Data Acquisition; 3.2 Experimental iSCAT Microscopy; 3.2.1 Image Processing; 3.2.2 Spot Detection; 3.2.3 Localisation; 3.2.4 Trajectory Linking
Text of Note
3.2.5 Assessment of Localisation Precision3.2.6 Self-referencing; References; 4 Anomalous Diffusion Due to Interleaflet Coupling and Molecular Pinning; 4.1 Introduction; 4.2 Experimental Methods; 4.2.1 Materials; 4.2.2 Vesicle Preparation; 4.2.3 Substrate Preparation; 4.2.4 Supported Lipid Bilayer Formation; 4.2.5 Instrument Setup Parameters; 4.3 Experimental Results; 4.3.1 GM1 Undergoes Anomalous Diffusion in Supported Lipid Bilayers; 4.3.2 Transient Confinement Causes Anomalous Diffusion; 4.3.3 Concentration Dependent Dynamics of Transient Binding
Text of Note
4.3.4 Recovery of Brownian Motion upon Tuning Substrate Interactions and Interleaflet Coupling4.4 Discussion; 4.4.1 Importance of Simultaneous Localisation Precision and Time Resolution; 4.4.2 Thermal and Optical Force Considerations; 4.4.3 Membrane Defects, Labelling Artefacts, and CTxB Induced Aggregation Do Not Cause Transient Binding; 4.4.4 Transient Binding Requires Substrate Interaction and Interleaflet Coupling; 4.4.5 Multiple CTxB-GM1 Interactions Result in Ring-Like Structures; 4.4.6 A Model of Transient Binding: Molecular Pinning; 4.5 Conclusion and Outlook; References
Text of Note
5 Structural Dynamics of Myosin 5a5.1 Introduction; 5.2 Experimental Methods; 5.2.1 Sample Preparation; 5.2.2 Experimental Setup; 5.3 Experimental Results; 5.3.1 N-Terminus Labelling Does Not Perturb the Kinetics of Myosin 5a; 5.3.2 During Myosin Movement the Motor Domain Undergoes a Transition Between Two Distinct States; 5.3.3 The Labelled Motor Domain Moves in Three Dimensions; 5.3.4 A Conformational Change in the Motor Domain Accompanies the Power Stroke; 5.3.5 Myosin Steps via a Single, Spatially-Constrained Transient State
0
8
8
8
8
SUMMARY OR ABSTRACT
Text of Note
This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physics to molecular biology and synthetic organic chemistry. Especially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field.
ACQUISITION INFORMATION NOTE
Source for Acquisition/Subscription Address
Springer Nature
Stock Number
com.springer.onix.9783319770956
OTHER EDITION IN ANOTHER MEDIUM
Title
Investigation of nanoscopic dynamics and potentials by interferometric scattering microscopy.
International Standard Book Number
9783319770949
TOPICAL NAME USED AS SUBJECT
Interferometry.
Microscopy.
Atomic & molecular physics.
Cellular biology (cytology)
Interferometry.
Microscopy.
NATURE-- Reference.
Organic chemistry.
Physical chemistry.
SCIENCE-- Life Sciences-- Biology.
SCIENCE-- Life Sciences-- General.
(SUBJECT CATEGORY (Provisional
NAT-- 027000
SCI-- 008000
SCI-- 086000
DEWEY DECIMAL CLASSIFICATION
Number
570
.
28/2
Edition
23
LIBRARY OF CONGRESS CLASSIFICATION
Class number
QH205
.
2
PERSONAL NAME - PRIMARY RESPONSIBILITY
Arroyo, Jaime Ortega
ORIGINATING SOURCE
Date of Transaction
20200823111741.0
Cataloguing Rules (Descriptive Conventions))
pn
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Book]
Y
